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ing contaminated. If you inject four sites, the culture has a .5 * .5 * .5 * .5 = 6% of NOT becoming contaminated. If you do everything right, this technique to increase your probability of producing a contamination (16 of 39) 5/1/2002 6:54:26 PM How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 4) free culture should not be necessary. However, many people have problems generating sterile spore prints at the start of their cultivating experience and this will help those people continue to generate cultures until they get enough experience. The first time you use a spore syringe that you prepared yourself, you may want to inoculate half of your jars the normal way, and the other half this way. If your spore syringe is just a 'little' dirty, this will give you second chance to grow more mushrooms and prepare a cleaner spore syringe. Disadvantages: It will take significantly longer for the jar to become 100% colonized. Back to preparation and colonization of substrate. table of contents. Adaptation-5: Large amounts of Inoculate. You can speed up the colonization of a jar dramatically by simply injecting the substrate material with more inoculate. If you inject 1 cc of inoculate at each site, you will get many germination's and the cake will colonize significantly faster. You should place the beveled side of the syringe needle against the glass so that the inoculate is coming out of the syringe and heading towards the glass. It should form a thin puddle of liquid between the glass and the substrate. 1 cc of inoculate should produce a puddle several inches in diameter. Advantage: Normally, people want the substrate to colonize as quickly as possible. This will help accomplish that goal. Also, the sooner and more fully the cake gets colonized, the less chance there is that contamination will get a foot hold and destroy the cake. (17 of 39) 5/1/2002 6:54:26 PM How To Grow Magic Mushrooms The Magic Mushroom Growers Guide (page 4) Disadvantages: This adaptation requires extra inoculate. If you are producing your own spore syringes it is not a factor. A single spore print can produce many (close to 50) spore syringes. If you are purchasing your spore syringes, you may wish to wait the few extra days to avoid the extra cost of using more inoculate. Back to preparation and colonization of substrate. table of contents. Adaptation-6: 80 Degree Colonization Temperature The culture jars can be colonized at a temperature higher than room temperature. 80 Degrees F. is ideal. Slightly higher is OK. There are several easy ways to accomplish this. If you have a floor heater with a pilot light and it is summer time (so the heat is not going to come on), you might be able to put the cakes in a shoe box and set them on the unit. The top of your water heater might be a good candidate. You can fill a cake pan half way with water and put a submersible fish tank heater in the water set to 80 degrees. Anadenanthera Colubrina Viable Seeds Terscheckii Cactus Skin Cuts (Trichocereus Terscheckii) Peganumharmala Melatonin Anadenanthera Colubrina Viable Seeds

crops is put into a 500 ml flask along with a solution made up of 150 ml ethyl alcohol, 150 ml water, and 100 grams KOH. Next, 15 ml of hydrazine hydrate is added. This hydrazine should be the monohydrate, which is 64% hydrazine. If a weaker variety has been scrounged up, this can be made to work by adding more, and using less water. Now the flask should be fitted with a condenser, and flushed with nitrogen. Then heat the flask in an oil bath to gentle boiling for 4 hours. A slow stream of nitrogen to the flask during the reflux averts the danger from hydrazine. The flask is next cooled, and the contents poured into a sep funnel of at least 1000 ml capacity. The batch is then extracted with 600 ml ether, followed by 600 ml of an 85-15% mix of ether and alcohol. Finally, one more extraction with 600 ml of 85-15% ether-alcohol is done. All of the desired product should now be extracted into the solvent, and out of the water. This fact should be checked using a black light to look for the characteristic blue fluorescence.
The combined solvent extracts should now be lowered to a pH of about 2 using HC1. At this point, a precipitate should form, and it should be filtered out. The precipitate should be washed free of entrained product with 4-1 ether-alcohol, and the washing added to the rest of the filtered solvent. Now 2750 ml of water should be added to

the solvent, and the mixture placed in a gallon and a half glass jug or 5000 ml beaker. To this should be added 3 portions of cation exchange resin in H* cycle. Cation exchange resin is a common item of commerce used in deionized water systems. Check the yellow pages under "water" and see which of the local Culligan men offer deionized water systems. The deionizers come in two-tank systems with one tank packed with cation exchange resin to remove calcium, magnesium and sodium from the water. The other tank has an anion exchange resin to remove chlorides, sulfates, and so on. It is no great task to buy cation exchange resin from these outlets. The resin consists of tiny plastic beads coated with the exchanger. In the case of the cation exchangers, Practical LSD Manufacture 44 this is generally a sulfonate.
"In H* cycle" means that the resin is charged up and ready to go. This is generally done by soaking the resin in 20% sulfuric acid in water for a while, then rinsing with distilled water. Check the directions on the container of resin. Steer clear of mixed resins that contain both anion and cation exchangers. If the Culligan man is too stupid to know the difference, or doesn't know what he has, keep looking until you find one who knows his business.
The treatment with three portions of cation exchange resin in H* cycle should be done Ayahuasca RED Vine (Banisteriopsis caapi)Collectors item, cm + in diameter!! as follows: Each portion of resin should weigh about 15 grams. The first portion is added, and then the mixture should be stirred strongly or shaken for about 10 Seeds Colubrina minutes. The product will come out of the liquid, and stick

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